ERCC5 Monoklonaler Antikörper

ERCC5 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G2C9

Kat-Nr. : 67055-1-PBS

Synonyme

XPG, 1G2C9, COFS3, DNA excision repair protein ERCC-5, DNA repair protein complementing XP-G cells

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  WB analysis using 67055-1-Ig (same clone as 67055-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 67055-1-Ig (same clone as 67055-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 67055-1-Ig (same clone as 67055-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67055-1-Ig (same clone as 67055-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67055-1-Ig (ERCC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67055-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67055-1-PBS bindet in WB, Indirect ELISA ERCC5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ERCC5 fusion protein Ag28561
• Vollständiger Name: excision repair cross-complementing rodent repair deficiency, complementation group 5
• Berechnetes Molekulargewicht: 1186 aa, 133 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC031522
• Gene symbol: ERCC5
• Gene ID (NCBI): 2073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677]. The calculated molecular weight of ERCC5 is 133 kDa, but the modified ERCC5 protein is about 200 kDa.