ERCC6L Rekombinanter Antikörper

ERCC6L Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

251669A8

Kat-Nr. : 86688-1-RR

Synonyme

ATP-dependent helicase ERCC6-like, DNA excision repair protein ERCC-6-like, EC:3.6.4.12, PICH, PLK1-interacting checkpoint helicase

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  WB analysis using 86688-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86688-1-RR (ERCC6L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 86688-1-RR

  IP result of anti-ERCC6L (IP:86688-1-RR, 4ug; Detection:86688-1-RR 1:2000) with K-562 cells lysate 1640 ug.

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  Affinity and Kinetic Characterization of 86688-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86688-1-RR against Human ERCC6L were performed. The affinity constant is 0.79 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86688-1-RR bindet in WB, IP, ELISA ERCC6L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ERCC6L fusion protein Ag8223
• Vollständiger Name: excision repair cross-complementing rodent repair deficiency, complementation group 6-like
• Berechnetes Molekulargewicht: 419 aa, 46 kDa, 141 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC008808
• Gene symbol: ERCC6L
• Gene ID (NCBI): 54821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERCC6L, also named as Tumor antigen BJ-HCC-15 or PICH, is a 1250 amino acid protein, which contains one helicase ATP-binding domain, one helicase C-terminal domain and two TPR repeats. ERCC6L localizes to kinetochores, inner centromeres and belongs to the SNF2/RAD54 helicase family. ERCC6L as a DNA helicase that acts as an essential component of the spindle assembly checkpoint. The calculated molecular weight of ERCC6L is 140 kDa, but the phosphorylated modification of molecular weight of ERCC6L is about 180kDa.