ERGIC2 Polyklonaler Antikörper

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ERGIC2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11927-1-PBS

Synonyme

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  WB analysis using 11927-1-AP (same clone as 11927-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11927-1-AP (ERGIC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11927-1-AP (same clone as 11927-1-PBS)

  WB result of ERGIC2 antibody (11927-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC2 transfected HeLa cells. This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 11927-1-AP (same clone as 11927-1-PBS)

  IP result of anti-ERGIC2 (IP:11927-1-AP, 4ug; Detection:11927-1-AP 1:1000) with HepG2 cells lysate 2900ug. This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11927-1-AP (same clone as 11927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11927-1-AP (same clone as 11927-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11927-1-AP (ERGIC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11927-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11927-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ERGIC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERGIC2 fusion protein Ag2603
• Vollständiger Name: ERGIC and golgi 2
• Berechnetes Molekulargewicht: 377 aa, 43 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC000887
• Gene symbol: ERGIC2
• Gene ID (NCBI): 51290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERGIC2, also named as Endoplasmic reticulum-Golgi intermediate compartment protein 2 or PTX1, is a 377 amino acid protein, which belongs to the ERGIC family. ERGIC2 is ubiquitously expressed in many tissues. ERGIC2 localizes in the endoplasmic reticulum-Golgi intermediate compartment membrane and may play a role in transport between endoplasmic reticulum and Golgi. ERGIC2 is involved in protein transportation, and participated in the elongation factor 1 alpha regulated mechanisms.