ERK1/2 Polyklonaler Antikörper

ERK1/2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51068-1-PBS

Synonyme

---

• 

  WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 51068-1-AP (same clone as 51068-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51068-1-AP (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51068-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51068-1-AP (ERK1/2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51068-1-AP (same clone as 51068-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51068-1-AP (ERK1/2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ERK1/2 antibody (51068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 51068-1-AP (same clone as 51068-1-PBS)

  Immunofluorescent analysis of HeLa cells using 51068-1-AP (ERK1/2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 51068-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

51068-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ERK1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase 1
• Berechnetes Molekulargewicht: 360 aa, 41 kDa
• Beobachtetes Molekulargewicht: 38-44 kDa
• GenBank-Zugangsnummer: BC017832
• Gene symbol: ERK2
• Gene ID (NCBI): 5594
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.