ERK1/2 Monoklonaler Antikörper

ERK1/2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(73)

CloneNo.

1A3D3

Kat-Nr. : 67170-1-Ig

Synonyme

ERK, ERK 1, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-12-Zellen, HeLa-Zellen, Jurkat-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
Erfolgreiche Detektion in IHChumanes Ovarialkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 71 publications below
IHCSee 1 publications below

Produktinformation

67170-1-Ig bindet in WB, IHC, ELISA ERK1/2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name mitogen-activated protein kinase 3
Berechnetes Molekulargewicht 38-43 kDa
GenBank-ZugangsnummerNM_002746
Gene symbol ERK1
Gene ID (NCBI) 5595
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ERK1/2 antibody 67170-1-IgProtokoll herunterladen
IHC protocol for ERK1/2 antibody 67170-1-IgProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Mol Cell

PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis

Authors - Shouqiao Hou
ratWB

J Hazard Mater

ERK/p38/ROS burst responses to environmentally relevant concentrations of diphenyl phosphate-evoked neutrophil extracellular traps formation: Assessing the role of autophagy.

Authors - Siwen Li
mouseWB

J Hazard Mater

Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders.

Authors - Tianxin Zhao
mouseWB

Cancer Lett

SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.

Authors - Tian-Jiao Li
humanWB

Int J Mol Sci

LMNA Reduced Acquired Resistance to Erlotinib in NSCLC by Reversing the Epithelial–Mesenchymal Transition via the FGFR/MAPK/c-fos Signaling Pathway

Authors - Chunsheng Hu
mouseWB

J Neuroinflammation

Regulatory coupling between long noncoding RNAs and senescence in irradiated microglia.

Authors - Anan Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

S (Verified Customer) (05-18-2020)

Excellent Antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: AC16
ERK1/2 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:67170-1-Ig)