ERK1/2 Monoklonaler Antikörper
ERK1/2 Monoklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, IHC, ELISA
Konjugation
Unkonjugiert
CloneNo.
1A3D3
Kat-Nr. : 67170-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | PC-12-Zellen, HeLa-Zellen, Jurkat-Zellen, RAW 264.7-Zellen, ROS1728-Zellen |
| Erfolgreiche Detektion in IHC | humanes Ovarialkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 71 publications below |
| IHC | See 1 publications below |
Produktinformation
67170-1-Ig bindet in WB, IHC, ELISA ERK1/2 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Kaninchen, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | mitogen-activated protein kinase 3 |
| Berechnetes Molekulargewicht | 38-43 kDa |
| GenBank-Zugangsnummer | NM_002746 |
| Gene symbol | ERK1 |
| Gene ID (NCBI) | 5595 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for ERK1/2 antibody 67170-1-Ig | Protokoll herunterladen |
| IHC protocol for ERK1/2 antibody 67170-1-Ig | Protokoll herunterladenl |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Hazard Mater ERK/p38/ROS burst responses to environmentally relevant concentrations of diphenyl phosphate-evoked neutrophil extracellular traps formation: Assessing the role of autophagy. | ||
J Hazard Mater Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders. | ||
Cancer Lett SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer. | ||
Int J Mol Sci LMNA Reduced Acquired Resistance to Erlotinib in NSCLC by Reversing the Epithelial–Mesenchymal Transition via the FGFR/MAPK/c-fos Signaling Pathway | ||
J Neuroinflammation Regulatory coupling between long noncoding RNAs and senescence in irradiated microglia. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH S (Verified Customer) (05-18-2020) | Excellent Antibody
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