ERK1/2 Rekombinanter Antikörper

ERK1/2 Rekombinant Antikörper für WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

4P8

Kat-Nr. : 83533-1-PBS

Synonyme

ERK, ERK1, ERK2, 4P8, EC:2.7.11.24

---

• 

  WB analysis using 83533-1-RR (same clone as 83533-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83533-1-RR (ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:100 (under 40x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83533-1-RR (same clone as 83533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83533-1-RR (ERK1/2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83533-1-RR (same clone as 83533-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ERK1/2 antibody (83533-1-RR, Clone: 4P8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83533-1-RR (same clone as 83533-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ERK1/2 Recombinant antibody (83533-1-RR, Clone:4P8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83533-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83533-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83533-1-RR against Human ERK1/2 were performed. The affinity constant is 17.8 nM.

---

Geprüfte Anwendungen

Produktinformation

83533-1-PBS bindet in WB, IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA ERK1/2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ERK1/2 fusion protein Ag20350
• Vollständiger Name: mitogen-activated protein kinase 3
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 38-44 kDa
• GenBank-Zugangsnummer: BC013992
• Gene symbol: ERK1
• Gene ID (NCBI): 5595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.