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Phospho-ERK1/2 (Thr202/Tyr204) Polyklonaler Antikörper
Phospho-ERK1/2 (Thr202/Tyr204) Polyklonal Antikörper für WB, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (5)
Anwendung
WB, IHC, IF, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 28733-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | NIH/3T3-Zellen, mit Calyculin A behandelte HEK-293T-Zellen |
Erfolgreiche IP | Mit Calyculin A behandelte PC-3-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 510 publications below |
IHC | See 30 publications below |
IF | See 19 publications below |
Produktinformation
28733-1-AP bindet in WB, IHC, IF, IP, ELISA Phospho-ERK1/2 (Thr202/Tyr204) und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte, Zebrafisch, Ziege |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | mitogen-activated protein kinase 3 |
Berechnetes Molekulargewicht | 38-43 kDa |
Beobachtetes Molekulargewicht | 38-43 kDa |
GenBank-Zugangsnummer | NM_002746 |
Gene symbol | ERK1 |
Gene ID (NCBI) | 5595 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. The antibody recognizes ERK2 phosphorylation sites Thr185 and Tyr187.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-AP | Protokoll herunterladen |
IP protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis | ||
Mol Cancer Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression. | ||
Biomaterials Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure | ||
Exp Mol Med Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer | ||
Redox Biol Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species | ||
EMBO Mol Med Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Charlotte (Verified Customer) (07-15-2024) | The last (most intense) band correspond to pERK. It worked nicely
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FH Alexandra (Verified Customer) (06-06-2023) | P19 cells were aggregated in bacteria petri dishes for 6h (point1) and 12 and 24 h (point 2 and 3) in the presence of 1uM RA (retinoic acid). For neuronal differentiation the RA treatment lasts for 4 days, the cells are trypsinized and cultured in serum free neurobasal medium supplemented with N2, glutamax and penstrep. Cell aggregates were collected by centrifugation, washed in 1X PBS and lysed in denaturing lysis buffer. For WB i used PVDF, blocking in 5%milk and the antibody incubation was O/N at 4C. Please do not include the Parp1 KO annotation if you will use this data. In the PDF you can find the ladder and the exposition as well. If the data did not get uploaded (i cannot tell) let me know and i will send it by email.
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FH Macarena Lucia (Verified Customer) (10-17-2022) | nice band
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