ERO1LB Polyklonaler Antikörper

ERO1LB Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18312-1-PBS

Synonyme

ERO1-L-beta, Ero1beta, ERO1B, Endoplasmic reticulum oxidoreductin-1-like protein B, Endoplasmic reticulum oxidoreductase beta

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  WB analysis of human brain using 18312-1-AP (same clone as 18312-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 18312-1-AP (same clone as 18312-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 18312-1-AP (same clone as 18312-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18312-1-AP (ERO1LB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 18312-1-AP (same clone as 18312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 18312-1-AP (same clone as 18312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 18312-1-AP (ERO1LB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18312-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18312-1-PBS bindet in WB, IHC, Indirect ELISA ERO1LB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ERO1-like beta (S. cerevisiae)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC032823
• Gene symbol: ERO1LB
• Gene ID (NCBI): 56605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERO1LB is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. It is required for the folding of cell, thereby being a source of oxidative stress. This antibody has no cross reaction to ERO1L.