ERP29 Monoklonaler Antikörper

ERP29 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H8B2

Kat-Nr. : 67675-1-PBS

Synonyme

C12orf8, ERp28, ERP29, ERp31, PDI DB, PDIA9

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  WB analysis using 67675-1-Ig (same clone as 67675-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67675-1-Ig (ERP29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67675-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA ERP29 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ERP29 fusion protein Ag30262
• Vollständiger Name: endoplasmic reticulum protein 29
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC101495
• Gene symbol: ERP29
• Gene ID (NCBI): 10961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).