ERP44 Monoklonaler Antikörper

ERP44 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

3A2C1

Kat-Nr. : 67426-1-Ig

Synonyme

ER protein 44, ERP44, KIAA0573, PDIA10, TXNDC4

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Galerie der Validierungsdaten

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  WB analysis using 67426-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67426-1-Ig (ERP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67426-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67426-1-Ig (ERP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67426-1-Ig bindet in WB, IHC,ELISA ERP44 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ERP44 fusion protein Ag8818
• Vollständiger Name: endoplasmic reticulum protein 44
• Berechnetes Molekulargewicht: 406 aa, 47 kDa
• Beobachtetes Molekulargewicht: 44-47 kDa
• GenBank-Zugangsnummer: BC005374
• Gene symbol: ERP44
• Gene ID (NCBI): 23071
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.