ERp57/ERp60 Monoklonaler Antikörper

ERp57/ERp60 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G4G7

Kat-Nr. : 66423-1-PBS

Synonyme

ERp57, PDIA3, 3G4G7, 58 kDa glucose-regulated protein, Disulfide isomerase ER-60

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  WB analysis using 66423-1-Ig (same clone as 66423-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66423-1-Ig (same clone as 66423-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66423-1-Ig (same clone as 66423-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66423-1-Ig (same clone as 66423-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66423-1-Ig (same clone as 66423-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66423-1-Ig (same clone as 66423-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66423-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66423-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ERp57/ERp60 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ERp57/ERp60 fusion protein Ag8741
• Vollständiger Name: protein disulfide isomerase family A, member 3
• Berechnetes Molekulargewicht: 505 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa, 60 kDa
• GenBank-Zugangsnummer: BC014433
• Gene symbol: PDIA3
• Gene ID (NCBI): 2923
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.