ERp72 Monoklonaler Antikörper

ERp72 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

1D5F3

Kat-Nr. : 66365-1-Ig

Synonyme

Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERp72, PDIA4, Protein disulfide isomerase A4

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  WB analysis using 66365-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66365-1-Ig (ERp72 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IHC staining of human liver cancer using 66365-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66365-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66365-1-Ig (ERp72 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, JAR-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 5 publications below

Produktinformation

66365-1-Ig bindet in WB, IHC, IF, ELISA ERp72 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ERp72 fusion protein Ag6873
• Vollständiger Name: protein disulfide isomerase family A, member 4
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC000425
• Gene symbol: ERp72
• Gene ID (NCBI): 9601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA4 (Protein disulfide-isomerase A4) is also named ERP70, ERP72, and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full-length protein has a signal peptide with 20 amino acids.

Publikationen

Species	Application	Title
human	WB	Cell Rep; The ubiquitin E3 ligase TRIM10 promotes STING aggregation and activation in the Golgi apparatus; Authors - Lingli Kong
human	IF	Hypertension; Upregulation of Piezo1 (Piezo Type Mechanosensitive Ion Channel Component 1) Enhances the Intracellular Free Calcium in Pulmonary Arterial Smooth Muscle Cells From Idiopathic Pulmonary Arterial Hypertension Patients.; Authors - Jing Liao
mouse	IF	Front Mol Biosci; Glycosylation at Asn254 Is Required for the Activation of the PDGF-C Protein.; Authors - Wenjie Hu
human	IF	J Exp Clin Cancer Res; 14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.; Authors - Jia Song
human,rat	IF	Biomed Pharmacother; AKAP1 alleviates VSMC phenotypic modulation and neointima formation by inhibiting Drp1-dependent mitochondrial fission; Authors - Jingwen Sun
human	IF	Brain; Heterozygous variants in USP25 cause genetic generalized epilepsy; Authors - Cui-Xia Fan