ESD Polyklonaler Antikörper

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• KD/KO Validated

ESD Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15244-1-AP

Synonyme

S-formylglutathione hydrolase, Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12

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  WB analysis of HAP1 using 15244-1-AP

  WB result of ESD antibody (15244-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and ESD knockout HAP1 cells.

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  WB analysis of K-562 using 15244-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15244-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15244-1-AP (ESD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HAP1 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15244-1-AP bindet in WB, ELISA ESD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ESD fusion protein Ag7457
• Vollständiger Name: esterase D/formylglutathione hydrolase
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC001169
• Gene symbol: ESD
• Gene ID (NCBI): 2098
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.