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ESD Monoklonaler Antikörper

ESD Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F5E1

Kat-Nr. : 67069-2-PBS

Synonyme

Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56

Filter:
  • Western Blot (WB) analysis of various lysates using ESD Monoclonal antibody (67069-2-Ig)

    WB analysis using 67069-2-Ig (same clone as 67069-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67069-2-Ig (ESD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67069-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ESD Monoclonal antibody (67069-2-Ig)

    IF Staining of HEK-293 using 67069-2-Ig (same clone as 67069-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ESD antibody (67069-2-Ig, Clone: 2F5E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67069-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67069-2-PBS bindet in WB, IF/ICC, ELISA ESD und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ESD fusion protein Ag7487
Vollständiger Name esterase D/formylglutathione hydrolase
Berechnetes Molekulargewicht 31 kDa
Beobachtetes Molekulargewicht 30-34 kDa
GenBank-ZugangsnummerBC001169
Gene symbol ESD
Gene ID (NCBI) 2098
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.