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ESM1 Polyklonaler Antikörper

ESM1 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12355-1-PBS

Synonyme

endocan, endothelial cell molecule 1, Endothelial cell specific molecule 1, Endothelial cell-specific molecule 1, ESM-1

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  • Western Blot (WB) analysis of HUVEC cells using ESM1 Polyclonal antibody (12355-1-AP)

    WB analysis of HUVEC using 12355-1-AP (same clone as 12355-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 12355-1-AP (ESM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12355-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HUVEC cells using ESM1 Polyclonal antibody (12355-1-AP)

    IP experiment of HUVEC using 12355-1-AP (same clone as 12355-1-PBS)

    IP result of anti-ESM1 (IP:12355-1-AP, 4ug; Detection:12355-1-AP 1:300) with HUVEC cells lysate 640 ug. This data was developed using the same antibody clone with 12355-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12355-1-PBS bindet in WB, IP, Indirect ELISA ESM1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ESM1 fusion protein Ag3019
Vollständiger Name endothelial cell-specific molecule 1
Berechnetes Molekulargewicht 184 aa, 20 kDa
Beobachtetes Molekulargewicht14 kDa
GenBank-ZugangsnummerBC011989
Gene symbol ESM1
Gene ID (NCBI) 11082
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ESM1 is a secreted protein mainly expressed in the endothelial cells. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue was restricted to lung.