ESM1 Rekombinanter Antikörper

ESM1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250287H3

Kat-Nr. : 86027-1-RR

Synonyme

endocan, endothelial cell molecule 1, Endothelial cell specific molecule 1, Endothelial cell-specific molecule 1, ESM-1

---

• 

  WB analysis using 86027-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86027-1-RR (ESM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86027-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86027-1-RR against Human ESM1 were performed. The affinity constant is 66.5 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, A2780-Zellen, DU 145-Zellen, Maushirngewebe, PC-3-Zellen, Rattenhirngewebe, SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86027-1-RR bindet in WB, ELISA ESM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ESM1 fusion protein Ag3019
• Vollständiger Name: endothelial cell-specific molecule 1
• Berechnetes Molekulargewicht: 184 aa, 20 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC011989
• Gene symbol: ESM1
• Gene ID (NCBI): 11082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ESM1, also known as endocan or endothelial cell-specific molecule 1, is a protein-coding gene that encodes a secreted proteoglycan primarily expressed in endothelial cells of human tissues, including lung, kidney, and vascular systems. The protein consists of a 20 kDa core protein with a dermatan sulfate chain, forming a 50 kDa mature proteoglycan. ESM1 plays a crucial role in endothelial cell proliferation, migration, and angiogenesis (blood vessel formation). It modulates vascular development and maintains endothelial homeostasis. Overexpression of ESM1 is observed in cancers such as gastric, cervical, and lung tumors, where it promotes angiogenesis and tumor progression via pathways like VEGF/ERK signaling. Elevated serum ESM1 levels are associated with poor clinical outcomes in cancer patients.