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ESPL1 Polyklonaler Antikörper

ESPL1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22261-1-PBS

Synonyme

Caspase like protein ESPL1, ESP1, KIAA0165, SEPARASE, SEPARIN

Filter:
  • Western Blot (WB) analysis of various lysates using ESPL1 Polyclonal antibody (22261-1-AP)

    WB analysis using 22261-1-AP (same clone as 22261-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22261-1-AP (ESPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22261-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

22261-1-PBS bindet in WB, Indirect ELISA ESPL1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name extra spindle pole bodies homolog 1 (S. cerevisiae)
Berechnetes Molekulargewicht 233 kDa
Beobachtetes Molekulargewicht170 kDa
GenBank-ZugangsnummerNM_012291
Gene symbol ESPL1
Gene ID (NCBI) 9700
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ESPL1 (Extra spindle poles-like 1 protein), also known as Separin, Separase, a cysteine endopeptidase, plays a vital role in the stable binding between sister chromatids before anaphase and their timely separation during anaphase, which is the key to chromosome inheritance (PMID: 34512713). ESPL1 expression is elevated and predicts poor outcomes in patients with various types of cancer, including breast cancer, glioma and endometrial cancer (PMID: 36465268). Full-length ESPL1 decreased after 30 min resulting in a ~ 170 kDa N-terminal (ESPL1N−term) and a ~ 60 kDa C-terminal polypeptide (ESPL1CC−term), reflecting ESPL1 activation and auto-cleavage (PMID: 30429481)