ER Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ER Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

110

Rezensionen (1)

Kat-Nr. : 21244-1-AP

Synonyme

ER, ER alpha, Era, ERα, ESR, ESR1, ESRA, Estradiol receptor, Estrogen receptor, estrogen receptor 1, NR3A1

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Galerie der Validierungsdaten

• 

  WB analysis using 21244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21244-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of NIH/3T3 using 21244-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 21244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 21244-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 21244-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21244-1-AP (ER antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 21244-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21244-1-AP (ER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushodengewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 81 publications below
• IF: See 16 publications below
• CoIP: See 1 publications below

Produktinformation

21244-1-AP bindet in WB, IHC, IF, CoIP, ELISA ER und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ER fusion protein Ag15738
• Vollständiger Name: estrogen receptor 1
• Berechnetes Molekulargewicht: 595 aa, 66 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC128573
• Gene symbol: ESR1
• Gene ID (NCBI): 2099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII; Authors - Xavier Rambout
mouse	WB	J Biomed Sci; Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.; Authors - Chunfang Zhang
mouse	WB	J Hazard Mater; Cyanidin-3-O-glucoside ameliorates cadmium induced uterine epithelium proliferation in mice.; Authors - Dacheng Yang
mouse	WB	Environ Pollut; Betulinic acid alleviates zearalenone-induced uterine injury in mice; Authors - Chenglin Yang
human	WB	J Med Chem; Discovery of Thieno[2,3-e]indazole Derivatives as Novel Oral Selective Estrogen Receptor Degraders with Highly Improved Antitumor Effect and Favorable Druggability.; Authors - Zhengyu Lu
mouse		Sci Rep; Tsc1 deficiency impairs mammary development in mice by suppression of AKT, nuclear ERα, and cell-cycle-driving proteins.; Authors - Zhenqi Qin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (05-07-2019)

There are multiple bands for this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF10A/MCF7

ER Antibody Western Blot, validation (1:1000 dilution) in MCF10A/MCF7 (Cat no:21244-1-AP)

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