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ESR2 Monoklonaler Antikörper

ESR2 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5D2C9

Kat-Nr. : 60197-1-PBS

Synonyme

ER BETA, Erb, ERβ, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using ESR2 Monoclonal antibody (60197-1-Ig)

    WB analysis of MCF-7 using 60197-1-Ig (same clone as 60197-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW 1990 cells using ESR2 Monoclonal antibody (60197-1-Ig)

    WB analysis of SW 1990 using 60197-1-Ig (same clone as 60197-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using ESR2 Monoclonal antibody (60197-1-Ig)

    WB analysis of human brain using 60197-1-Ig (same clone as 60197-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 60197-1-Ig (ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60197-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

60197-1-PBS bindet in WB, Indirect ELISA ESR2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ESR2 fusion protein Ag5103
Vollständiger Name estrogen receptor 2 (ER beta)
Berechnetes Molekulargewicht 59 kDa
Beobachtetes Molekulargewicht 50-60 kDa
GenBank-ZugangsnummerBC024181
Gene symbol ESR2
Gene ID (NCBI) 2100
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa (PMID: 14766008).