ESR2 Rekombinanter Antikörper

ESR2 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240412F2

Kat-Nr. : 83447-3-PBS

Synonyme

240412F2, ER BETA, Erb, ER-beta, ERβ

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  IHC staining of mouse testis using 83447-3-RR (same clone as 83447-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83447-3-RR (ESR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 83447-3-RR (same clone as 83447-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 83447-3-RR (ESR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83447-3-RR (same clone as 83447-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ESR2 antibody (83447-3-RR, Clone: 240412F2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83447-3-RR (same clone as 83447-3-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Esr2 Recombinant Antibody (83447-3-RR, Clone:240412F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83447-3-RR (same clone as 83447-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Esr2 Recombinant Antibody (83447-3-RR, Clone:240412F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83447-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83447-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83447-3-PBS against Human ESR2 was performed. The affinity constant is 16.6 pM.

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  Affinity and Kinetic Characterization of 83447-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83447-3-RR against Human ESR2 were performed. The affinity constant is 0.22 nM.

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Geprüfte Anwendungen

Produktinformation

83447-3-PBS bindet in IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA ESR2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ESR2 fusion protein Ag5103
• Vollständiger Name: estrogen receptor 2 (ER beta)
• Berechnetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC024181
• Gene symbol: ESR2
• Gene ID (NCBI): 2100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.