ESR2 Beta 3 Polyklonaler Antikörper

ESR2 Beta 3 Polyklonal Antikörper für IF, IHC,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55441-1-AP

Synonyme

ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 3, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

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Galerie der Validierungsdaten

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  IHC staining of human cervix using 55441-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 55441-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervix using 55441-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervix using 55441-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 55441-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 55441-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 55441-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 55441-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 55441-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ESR2 Beta 3 antibody (55441-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red) DAPI (blue).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Zervixgewebe, humanes Endometriumkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55441-1-AP bindet in IHC, IF,ELISA ESR2 Beta 3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: estrogen receptor 2 (ER beta)
• Berechnetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_001437
• Gene symbol: ESR2
• Gene ID (NCBI): 2100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual