ESRP2 Polyklonaler Antikörper

ESRP2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 23117-1-AP

Synonyme

ESRP2, RBM35B, RNA binding motif protein 35B, RNA binding protein 35B

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  WB analysis using 23117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23117-1-AP (ESRP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 23117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23117-1-AP (ESRP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 23117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23117-1-AP (ESRP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 23117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23117-1-AP (ESRP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 23117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23117-1-AP (ESRP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 23117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23117-1-AP (ESRP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23117-1-AP bindet in WB, IHC, ELISA ESRP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ESRP2 fusion protein Ag19436
• Vollständiger Name: epithelial splicing regulatory protein 2
• Berechnetes Molekulargewicht: 727 aa, 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC030146
• Gene symbol: ESRP2
• Gene ID (NCBI): 80004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ESRP2 (Epithelial Splicing Regulatory Protein 2), also known as RBM35B(RNA binding motif protein 35B), is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family of RNA binding proteins. ESRP2 plays a role in the regulation of alternative splicing events of pre-mRNAs(PMID: 27401076). Overexpression of ESRP2 has been described in various malignant tumors, such as pancreatic ductal adenocarcinoma, oral squamous carcinoma, ovarian cancer, and luminal-type breast cancer(PMID: 33339518).

Publikationen

Species	Application	Title
human		Oncogene; Androgen receptor signaling regulates the transcriptome of prostate cancer cells by modulating global alternative splicing.; Authors - Kalpit Shah