ESYT2 Monoklonaler Antikörper

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• KD/KO Validated

ESYT2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1B12G2

Kat-Nr. : 68374-1-Ig

Synonyme

FAM62B, Chr2Syt, E Syt2, E-Syt2, Extended synaptotagmin 2

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  WB analysis using 68374-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68374-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (ESYT2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68374-1-Ig

  WB result of FAM62B antibody (68374-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM62B transfected HeLa cells.

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  WB analysis using 68374-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 68374-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ESYT2 antibody (68374-1-Ig, Clone: 1B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HEK-293-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

68374-1-Ig bindet in WB, IF/ICC, ELISA ESYT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ESYT2 fusion protein Ag20440
• Vollständiger Name: family with sequence similarity 62 (C2 domain containing) member B
• Berechnetes Molekulargewicht: 921 aa, 102 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC013957
• Gene symbol: ESYT2
• Gene ID (NCBI): 57488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ESYT2, also named FAM62B, is one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Sphingosine-1-phosphate signalling activates E-Syt1 to facilitate HDL-derived cholesterol transport; Authors - Zizhen Xu ; KD Validated