ESYT2 Monoklonaler Antikörper

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ESYT2 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B12G2

Kat-Nr. : 68374-1-PBS

Synonyme

FAM62B, Chr2Syt, E Syt2, E-Syt2, Extended synaptotagmin 2

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  WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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  WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (ESYT2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68374-1-Ig (same clone as 68374-1-PBS)

  WB result of FAM62B antibody (68374-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM62B transfected HeLa cells. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68374-1-Ig (same clone as 68374-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ESYT2 antibody (68374-1-Ig, Clone: 1B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68374-1-PBS bindet in WB, IF/ICC, Indirect ELISA ESYT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ESYT2 fusion protein Ag20440
• Vollständiger Name: family with sequence similarity 62 (C2 domain containing) member B
• Berechnetes Molekulargewicht: 921 aa, 102 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC013957
• Gene symbol: ESYT2
• Gene ID (NCBI): 57488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ESYT2, also named FAM62B, is one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.