ETF1 Polyklonaler Antikörper

ETF1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 10884-1-AP

Synonyme

TB3-1, TB3 1, SUP45L1, RF1, ERF1

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  WB analysis using 10884-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10884-1-AP (ETF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10884-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10884-1-AP (ETF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10884-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10884-1-AP (ETF1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 10884-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10884-1-AP (ETF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10884-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10884-1-AP (ETF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10884-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ETF1 antibody (10884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: T-47D-Zellen, Maushodengewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

10884-1-AP bindet in WB, IHC, IF/ICC, ELISA ETF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ETF1 fusion protein Ag1330
• Vollständiger Name: eukaryotic translation termination factor 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC014269
• Gene symbol: ETF1
• Gene ID (NCBI): 2107
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome, which is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In eukaryotes a single class-1 translation termination factor eRF1 decodes the three stop codons: UAA, UAG and UGA. [PMID:7990965,20860996]

Publikationen

Species	Application	Title
human	IF	Cells; Relocalization of Translation Termination and Ribosome Recycling Factors to Stress Granules Coincides with Elevated Stop-Codon Readthrough and Reinitiation Rates upon Oxidative Stress; Authors - Desislava S Makeeva