ETFA Polyklonaler Antikörper

ETFA Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 12262-1-AP

Synonyme

Alpha ETF, Alpha-ETF, EMA, GA2, MADD

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  WB analysis using 12262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12262-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 12262-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human ileum using 12262-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12262-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12262-1-AP (ETFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12262-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12262-1-AP (ETFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12262-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETFA antibody (12262-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594phalloidin (red).

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  FC experiment of MCF-7 using 12262-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug ETFA Polyclonal antibody (12262-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, humanes Ileumgewebe, Maus-Dünndarmgewebe, Ratten-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

12262-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA ETFA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ETFA fusion protein Ag2931
• Vollständiger Name: electron-transfer-flavoprotein, alpha polypeptide
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC015526
• Gene symbol: ETFA
• Gene ID (NCBI): 2108
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kD alpha subunits (ETFA) and 28 kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. ETFA serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.

Publikationen

Species	Application	Title
human	IF	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
mouse	WB	Cell Mol Life Sci; Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.; Authors - Min-Zhi Peng
human	WB	Bioorg Chem; Pomalidomide sensitizes lung cancer cells to TRAIL/CDDP-induced apoptosis via directly targeting electron transfer flavoprotein alpha subunit; Authors - Liangping Li
human	WB	J Proteome Res; Proteomic analysis of nuclei isolated from cancer cell lines treated with indenoisoquinoline NSC 724998, a novel topoisomerase I inhibitor.; Authors - Han Bingnan B
human	WB	J Proteomics; Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis.; Authors - Muto Taika T