ETFA Polyklonaler Antikörper

ETFA Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12262-1-PBS

Synonyme

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  WB analysis using 12262-1-AP (same clone as 12262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

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  WB analysis of human ileum using 12262-1-AP (same clone as 12262-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 12262-1-AP (same clone as 12262-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 12262-1-AP (same clone as 12262-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12262-1-AP (same clone as 12262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12262-1-AP (ETFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12262-1-AP (same clone as 12262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12262-1-AP (ETFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12262-1-AP (same clone as 12262-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETFA antibody (12262-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594phalloidin (red). This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 12262-1-AP (same clone as 12262-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug ETFA Polyclonal antibody (12262-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12262-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA ETFA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ETFA fusion protein Ag2931
• Vollständiger Name: electron-transfer-flavoprotein, alpha polypeptide
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC015526
• Gene symbol: ETFA
• Gene ID (NCBI): 2108
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kD alpha subunits (ETFA) and 28 kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. ETFA serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.