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ETS2 Rekombinanter Antikörper

ETS2 Rekombinant Antikörper für IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242817H8

Kat-Nr. : 85113-4-PBS

Synonyme

242817H8, ETS-2, ETS2IT1, Protein C ets 2, Protein C-ets-2

Filter:
  • Sandwich ELISA standard curve of MP01858-3

    Sandwich ELISA standard curve of MP01858-3

    Sandwich ELISA standard curve of MP01858-3, Human ETS2 Recombinant Matched Antibody Pair - PBS only. 85113-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2929. 85113-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP01858-3

    Sample test of MP01858-3

    The mean ETS2 concentration was determined to be 26.79 ng/mL in K-562 cell extract based on a 2.0 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ETS2 Recombinant antibody (85113-4-RR)

    IF Staining of HeLa using 85113-4-RR (same clone as 85113-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85113-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ETS2 Recombinant antibody (85113-4-RR)

    IF Staining of MCF-7 using 85113-4-RR (same clone as 85113-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85113-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using ETS2 Recombinant antibody (85113-4-RR)

    FC experiment of MCF-7 using 85113-4-RR (same clone as 85113-4-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ETS2 Recombinant antibody (85113-4-RR, Clone:242817H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85113-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85113-4-RR

    Affinity and Kinetic Characterization of 85113-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85113-4-RR against Human ETS2 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85113-4-PBS bindet in IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test ETS2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ETS2 fusion protein Ag2929
Vollständiger Name v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)
Berechnetes Molekulargewicht 469 aa, 53 kDa
GenBank-ZugangsnummerBC017040
Gene symbol ETS2
Gene ID (NCBI) 2114
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.