ETS2 Rekombinanter Antikörper

ETS2 Rekombinant Antikörper für IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242817H8

Kat-Nr. : 85113-4-RR

Synonyme

242817H8, ETS-2, ETS2IT1, Protein C ets 2, Protein C-ets-2

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  IF Staining of HeLa using 85113-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 85113-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ETS2 antibody (85113-4-RR, Clone: 242817H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of MCF-7 using 85113-4-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ETS2 Recombinant antibody (85113-4-RR, Clone:242817H8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 85113-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85113-4-RR against Human ETS2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85113-4-RR bindet in IF/ICC, FC (Intra), ELISA ETS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ETS2 fusion protein Ag2929
• Vollständiger Name: v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)
• Berechnetes Molekulargewicht: 469 aa, 53 kDa
• GenBank-Zugangsnummer: BC017040
• Gene symbol: ETS2
• Gene ID (NCBI): 2114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.