ETV4 Polyklonaler Antikörper

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• KD/KO Validated

ETV4 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 10684-1-AP

Synonyme

E1A F, E1AF, ETS translocation variant 4, ets variant 4, ETV4, PEA3, PEAS3, Protein PEA3

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 10684-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10684-1-AP (ETV4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10684-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10684-1-AP (ETV4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10684-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10684-1-AP (ETV4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 10684-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10684-1-AP (ETV4 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

10684-1-AP bindet in WB, IHC, IF, ELISA ETV4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ETV4 fusion protein Ag0984
• Vollständiger Name: ets variant 4
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC007242
• Gene symbol: ETV4
• Gene ID (NCBI): 2118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.

Publikationen

Species	Application	Title
human	WB	Cell; Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription.; Authors - Jaehong Suh
mouse	WB	Dev Cell; ATXN1 protein family and CIC regulate extracellular matrix remodeling and lung alveolarization.; Authors - Lee Yoontae Y ; KD Validated
mouse	WB	Nat Commun; Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5.; Authors - Sungjun Park
human	WB	Cancer Res; Targeting pan-ETS factors inhibits melanoma progression.; Authors - Lee Huang
human,mouse	WB	Proc Natl Acad Sci U S A; Erk1/2 inactivation promotes a rapid redistribution of COP1 and degradation of COP1 substrates.; Authors - Weiming Ouyang
human	WB	Oncogene; HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer; Authors - Bei Liu ; KD Validated