ETV5 Polyklonaler Antikörper

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• KD/KO Validated

ETV5 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13011-1-PBS

Synonyme

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  WB analysis using 13011-1-AP (same clone as 13011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13011-1-AP (same clone as 13011-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13011-1-AP (ETV5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13011-1-AP (same clone as 13011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13011-1-AP (same clone as 13011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13011-1-AP (ETV5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 13011-1-AP (same clone as 13011-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 13011-1-AP (ETV5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13011-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13011-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ETV5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ETV5 fusion protein Ag3708
• Vollständiger Name: ets variant 5
• Berechnetes Molekulargewicht: 510 aa, 58 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC007333
• Gene symbol: ETV5
• Gene ID (NCBI): 2119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney