EVI1 Polyklonaler Antikörper

EVI1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 23201-1-AP

Synonyme

MECOM, MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367

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  WB analysis of SKOV-3 using 23201-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 23201-1-AP (EVI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 23201-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 23201-1-AP (EVI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 23201-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 23201-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 23201-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 23201-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using 23201-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using EVI1 antibody (23201-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

23201-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA EVI1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EVI1 fusion protein Ag19603
• Vollständiger Name: ecotropic viral integration site 1
• Berechnetes Molekulargewicht: 1239 aa, 139 kDa
• Beobachtetes Molekulargewicht: 118 kDa
• GenBank-Zugangsnummer: BC130520
• Gene symbol: EVI1
• Gene ID (NCBI): 2122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Sci Adv; Predicting master transcription factors from pan-cancer expression data.; Authors - Jessica Reddy
human	CoIP	Oncol Rep; Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis; Authors - Zhitao Chen