EVI2B Rekombinanter Antikörper

EVI2B Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250344C9

Kat-Nr. : 85943-1-RR

Synonyme

CD361, D17S376, EVDB, EVI 2B, EVI-2B

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  WB analysis using 85943-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85943-1-RR (EVI2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 85943-1-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using EVI2B antibody (85943-1-RR, Clone: 250344C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen, HL-60-Zellen, Ramos-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85943-1-RR bindet in WB, IF/ICC, ELISA EVI2B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EVI2B fusion protein Eg3709
• Vollständiger Name: ecotropic viral integration site 2B
• Berechnetes Molekulargewicht: 448 aa, 49 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC005926
• Gene symbol: EVI2B
• Gene ID (NCBI): 2124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ecotropic viral integration site 2B protein (EVI2B) is a type I transmembrane protein embedded within intron 27b of the neurofibromatosis type 1 (NF1) gene. It is transcribed in the opposite direction to the NF1 gene (PMID: 37584733). EVI2B is a downstream target of CCAAT/enhancer binding protein alpha (C/EBPα), which is crucial for myeloid differentiation and the function of hematopoietic progenitors (PMID: 36830696). This protein is highly expressed in mature granulocytes and is involved in the regulation of immune cell maturation (PMID: 28186500).