EVI5 Rekombinanter Antikörper

EVI5 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241918D11

Kat-Nr. : 84738-2-PBS

Synonyme

241918D11, NB4S

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  IHC staining of mouse stomach using 84738-2-RR (same clone as 84738-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84738-2-RR (EVI5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84738-2-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84738-2-RR (same clone as 84738-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EVI5 antibody (84738-2-RR, Clone: 241918D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84738-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84738-2-RR (same clone as 84738-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug EVI5 Recombinant antibody (84738-2-RR, Clone:241918D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84738-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84738-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84738-2-RR against Human EVI5 were performed. The affinity constant is 1.11 nM.

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Geprüfte Anwendungen

Produktinformation

84738-2-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA EVI5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EVI5 fusion protein Ag26739
• Vollständiger Name: ecotropic viral integration site 5
• Berechnetes Molekulargewicht: 810 aa, 93 kDa
• GenBank-Zugangsnummer: BC156793
• Gene symbol: EVI5
• Gene ID (NCBI): 7813
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EVI5 functions as a regulator of cell cycle progression by stabilizing the FBXO5 protein and promoting cyclin-A accumulation during interphase. It may play a role in cytokinesis (PMID: 16439210).