EVL Polyklonaler Antikörper

EVL Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13484-1-PBS

Synonyme

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  WB analysis of Jurkat using 13484-1-AP (same clone as 13484-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13484-1-AP (EVL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 13484-1-AP (same clone as 13484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 13484-1-AP (same clone as 13484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 13484-1-AP (same clone as 13484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 13484-1-AP (same clone as 13484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13484-1-PBS bindet in WB, IHC, Indirect ELISA EVL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EVL fusion protein Ag4302
• Vollständiger Name: Enah/Vasp-like
• Berechnetes Molekulargewicht: 418 aa, 45 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC032358
• Gene symbol: EVL
• Gene ID (NCBI): 51466
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EVL (Ena/VASP-like protein) is implicated in processes that requires dynamic actin remodeling such as axon guidance and platelet activation.It has two isoforms, EVL and EVL-I,that are highly expressed in hematopoietic cells of thymus and spleen,and both isoforms can be phosphorylated by PKA in vitro(PMID10945997).