EWS Polyklonaler Antikörper

EWS Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55191-1-PBS

Synonyme

EWSR1, EWS oncogene, RNA binding protein EWS, RNA-binding protein EWS

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  WB analysis using 55191-1-AP (same clone as 55191-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 55191-1-AP (same clone as 55191-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55191-1-AP (same clone as 55191-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55191-1-AP (EWS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 55191-1-AP (same clone as 55191-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55191-1-AP (EWS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55191-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55191-1-PBS bindet in WB, IHC, Indirect ELISA EWS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Ewing sarcoma breakpoint region 1
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: NM_005243
• Gene symbol: EWS
• Gene ID (NCBI): 2130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EWS, also named as WSR1, belongs to the RRM TET family. It might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. This antibody is specific to EWS. And a 90 kDa band also could be detected (PMID:23552423).