EXD2 Polyklonaler Antikörper

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• KD/KO Validated

EXD2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20138-1-PBS

Synonyme

3'-5' exoribonuclease EXD2, C14orf114, EC:3.1.11.1, EC:3.1.13.-, EXDL2

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  WB analysis of mouse lung using 20138-1-AP (same clone as 20138-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20138-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 20138-1-AP (same clone as 20138-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20138-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20138-1-AP (same clone as 20138-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20138-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 20138-1-AP (same clone as 20138-1-PBS)

  IP result of anti-EXD2 (IP:20138-1-AP, 3ug; Detection:20138-1-AP 1:300) with mouse lung tissue lysate 5160ug. This data was developed using the same antibody clone with 20138-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 20138-1-AP (same clone as 20138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20138-1-AP (EXD2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20138-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 20138-1-AP (same clone as 20138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20138-1-AP (EXD2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20138-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20138-1-AP (same clone as 20138-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EXD2 antibody (20138-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20138-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20138-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA EXD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXD2 fusion protein Ag14090
• Vollständiger Name: exonuclease 3'-5' domain containing 2
• Berechnetes Molekulargewicht: 70 kD aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC001962
• Gene symbol: EXD2
• Gene ID (NCBI): 55218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXD2 (Exonuclease 3'-5' Domain Containing 2), is a protein with exonuclease activity of 3'-5' nucleic acid, which is involved in many biological processes in cells, including DNA repair, RNA processing and mitochondrial translation. EXD2 protein is mainly located in mitochondrial outer membrane, intermediate fiber cytoskeleton and DNA damage site. This protein may be a potential drug target in some cancers. For example, in ALT (telomere selective elongation) dependent cancer, the co-elimination of EXD2 with BLM, DNA2 or POLD3 may mediate synthetic lethality. In addition, EXD2 inhibitors may exert anticancer effects by inhibiting mitochondrial translation and metabolic reprogramming. It has two isoforms with molecular weights of 56 kDa and 70 kDa respectively.