EXO1/Exonuclease 1 Polyklonaler Antikörper

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• KD/KO Validated

EXO1/Exonuclease 1 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16253-1-PBS

Synonyme

EXO1, EC:3.1.-.-, EXOI, Exonuclease 1, Exonuclease I

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  WB analysis using 16253-1-AP (same clone as 16253-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16253-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 16253-1-AP (same clone as 16253-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16253-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse thymus using 16253-1-AP (same clone as 16253-1-PBS)

  IP result of anti-EXO1 (IP:16253-1-AP, 4ug; Detection:16253-1-AP 1:500) with mouse thymus tissue lysate 2800ug. This data was developed using the same antibody clone with 16253-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16253-1-PBS bindet in WB, IP, Indirect ELISA EXO1/Exonuclease 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXO1/Exonuclease 1 fusion protein Ag9253
• Vollständiger Name: exonuclease 1
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 115-120 kDa
• GenBank-Zugangsnummer: BC007491
• Gene symbol: EXO1
• Gene ID (NCBI): 9156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.