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EXO1/Exonuclease 1 Rekombinanter Antikörper

EXO1/Exonuclease 1 Rekombinant Antikörper für WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251210D3

Kat-Nr. : 86445-1-PBS

Synonyme

EXO1, EC:3.1.-.-, EXOI, Exonuclease 1, Exonuclease I

Filter:
  • Western Blot (WB) analysis of various lysates using EXO1/Exonuclease 1 Recombinant monoclonal antibody (86445-1-RR)

    WB analysis using 86445-1-RR (same clone as 86445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86445-1-RR (EXO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86445-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02742-1

    Sandwich ELISA sample test of MP02742-1

    The mean EXO1/Exonuclease 1 concentration was determined to be 4.80 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02742-1

    Sandwich ELISA standard curve of MP02742-1

    Sandwich ELISA standard curve of MP02742-1, Human EXO1/Exonuclease 1 Recombinant Matched Antibody Pair - PBS only. 86445-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9253. 86445-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using EXO1/Exonuclease 1 Recombinant monoclonal antibody (86445-1-RR)

    IF Staining of HEK-293 using 86445-1-RR (same clone as 86445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EXO1 antibody (86445-1-RR, Clone: 251210D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86445-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86445-1-PBS bindet in WB, IF/ICC, Sandwich ELISA, Indirect ELISA EXO1/Exonuclease 1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen EXO1/Exonuclease 1 fusion protein Ag9253
Vollständiger Name exonuclease 1
Berechnetes Molekulargewicht 94 kDa
Beobachtetes Molekulargewicht 115-120 kDa
GenBank-ZugangsnummerBC007491
Gene symbol EXO1
Gene ID (NCBI) 9156
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.