EXOC1 Rekombinanter Antikörper

EXOC1 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241058D6

Kat-Nr. : 84007-5-PBS

Synonyme

241058D6, BM 102, Exocyst complex component 1, Exocyst complex component Sec3, SEC3

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  WB analysis using 84007-5-RR (same clone as 84007-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84007-5-RR (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84007-5-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84007-5-RR (same clone as 84007-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84007-5-RR (EXOC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84007-5-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 84007-5-RR (same clone as 84007-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (84007-5-RR, Clone: 241058D6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84007-5-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 84007-5-RR (same clone as 84007-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (84007-5-RR, Clone: 241058D6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84007-5-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 84007-5-RR (same clone as 84007-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (84007-5-RR, Clone: 241058D6 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84007-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84007-5-PBS bindet in WB, IHC, IF-P, Indirect ELISA EXOC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EXOC1 fusion protein Ag2303
• Vollständiger Name: exocyst complex component 1
• Berechnetes Molekulargewicht: 894 aa, 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC020650
• Gene symbol: EXOC1
• Gene ID (NCBI): 55763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXOC1 (exocyst complex component 1), also known as SEC3, is a component of the exocyst complex which is essential for the targeting of exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex is an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation.Recently it has been reported that exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion, which suggests a new paradigm of exocyst involvement in polarized targeting for intercellular transfer of viral proteins and viruses.