EXOC5 Polyklonaler Antikörper

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• KD/KO Validated

EXOC5 Polyklonal Antikörper für IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17593-1-PBS

Synonyme

Exocyst complex component 5, Exocyst complex component Sec10, hSec10, SEC10, SEC10L1

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  WB analysis using 17593-1-AP (same clone as 17593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17593-1-AP (EXOC5 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17593-1-AP (same clone as 17593-1-PBS)

  IP result of anti-EXOC5 (IP:17593-1-AP, 3ug; Detection:17593-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17593-1-AP (same clone as 17593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17593-1-AP (same clone as 17593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 17593-1-AP (same clone as 17593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 17593-1-AP (same clone as 17593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17593-1-AP (EXOC5 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17593-1-AP (same clone as 17593-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using EXOC5 antibody 17593-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 17593-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17593-1-PBS bindet in IHC, IF/ICC, IP, Indirect ELISA EXOC5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOC5 fusion protein Ag11524
• Vollständiger Name: exocyst complex component 5
• Berechnetes Molekulargewicht: 708 aa, 82 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC041126
• Gene symbol: EXOC5
• Gene ID (NCBI): 10640
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXOC5 (SEC10), a component of the exocyst complex, is a 77-kDa protein with a broad tissue distribution (PubMed: 9119050). The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The complex is also essential for the biogenesis of epithelial cell surface polarity.