EXOSC2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EXOSC2 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 14805-1-AP

Synonyme

EXOSC2, exosome component 2, hRrp4p, p7, RRP4, Rrp4p

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 14805-1-AP

  WB result of EXOSC2 antibody (14805-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC2 transfected HEK-293 cells.

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  WB analysis of HeLa using 14805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14805-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14805-1-AP

  IP Result of anti-EXOSC2 (IP:14805-1-AP, 4ug; Detection:14805-1-AP 1:1000) with HeLa cells lysate 1080ug.

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  IHC staining of human skin cancer using 14805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin cancer using 14805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 14805-1-AP

  Immunofluorescent analysis of MCF-7 cells, using EXOSC2 antibody 14805-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

14805-1-AP bindet in WB, IP, IHC, IF, ELISA EXOSC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOSC2 fusion protein Ag6525
• Vollständiger Name: exosome component 2
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC000747
• Gene symbol: EXOSC2
• Gene ID (NCBI): 23404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].

Publikationen

Species	Application	Title
human	WB,IF	Cell; Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.; Authors - Hani Goodarzi
human		Cell; The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.; Authors - Jakob Trendel
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
human	WB	Mol Biotechnol; EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal; Authors - Chen-Guang Lv ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (12-04-2022)

50µg of total protein were loaded on a gel. Incubation ON at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:14805-1-AP)

FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:14805-1-AP)