EXOSC3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EXOSC3 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (2)

Kat-Nr. : 15062-1-AP

Synonyme

CGI 102, EXOSC3, exosome component 3, hRrp 40, hRrp40p, p10, RP11 3J10.8, RRP40, Rrp40p

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Galerie der Validierungsdaten

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  WB analysis using 15062-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15062-1-AP (EXOSC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15062-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15062-1-AP (EXOSC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 15062-1-AP

  IP Result of anti-EXOSC3 (IP:15062-1-AP, 4ug; Detection:15062-1-AP 1:500) with A2780 cells lysate 800ug.

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  IHC staining of human colon cancer using 15062-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15062-1-AP (EXOSC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15062-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15062-1-AP (EXOSC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 15062-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using EXOSC3 antibody (15062-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, HEK-293T-Zellen, Mausmilzgewebe, NIH/3T3-Zellen, PC-3-Zellen
• Erfolgreiche IP: A2780-Zellen
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 2 publications below

Produktinformation

15062-1-AP bindet in WB, IP, IF, ELISA EXOSC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOSC3 fusion protein Ag7065
• Vollständiger Name: exosome component 3
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC002437
• Gene symbol: EXOSC3
• Gene ID (NCBI): 51010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA exosomes are multi-subunit complexes conserved throughout evolution, and they are emerging as the major cellular machinery for processing, surveillance and turnover of a diverse spectrum of coding and noncoding RNA substrates essential for viability[PMID:22544365]. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts [PMID:11782436]. EXOSC3 is a non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of Rnase PH-domain subunits through contacts with EXOSC9 and EXOSC5 [PMID:21255825].

Publikationen

Species	Application	Title
human		Mol Cell; Integrator is a genome-wide attenuator of non-productive transcription.; Authors - Søren Lykke-Andersen ; KD Validated
mouse	WB	Nucleic Acids Res; The RNA exosome contributes to gene expression regulation during stem cell differentiation.; Authors - Marta Lloret-Llinares
human	WB	Nucleic Acids Res; The RNA exosome shapes the expression of key protein-coding genes.; Authors - Mengjun Wu
human	WB	Nucleic Acids Res; A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.; Authors - Katarzyna Kalisiak
human	WB	Nat Commun; EXOSC8 mutations alter mRNA metabolism and cause hypomyelination with spinal muscular atrophy and cerebellar hypoplasia.; Authors - Veronika Boczonadi
human	WB	Nucleic Acids Res; A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.; Authors - Katarzyna Kalisiak

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (06-01-2022)

Comparison of WT and Exosc3 depletion showed a reduction in Exosc3 protein levels (confirming the specificity of the antibodies). Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC3 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:15062-1-AP)

FH

Nikolaus (Verified Customer) (05-24-2022)

Exosc3 conditional KO timecourse; Hsp70 as loading control; exoscenllent abs

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: mESC

EXOSC3 Antibody Western Blot validation (1/5000 dilution) in mESC (Cat no:15062-1-AP)