EXOSC3 Polyklonaler Antikörper

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• KD/KO Validated

EXOSC3 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15062-1-PBS

Synonyme

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  WB analysis using 15062-1-AP (same clone as 15062-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15062-1-AP (EXOSC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15062-1-AP (same clone as 15062-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15062-1-AP (EXOSC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A2780 using 15062-1-AP (same clone as 15062-1-PBS)

  IP result of anti-EXOSC3 (IP:15062-1-AP, 4ug; Detection:15062-1-AP 1:500) with A2780 cells lysate 800ug. This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15062-1-AP (same clone as 15062-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15062-1-AP (EXOSC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15062-1-AP (same clone as 15062-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15062-1-AP (EXOSC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 15062-1-AP (same clone as 15062-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using EXOSC3 antibody (15062-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15062-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA EXOSC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOSC3 fusion protein Ag7065
• Vollständiger Name: exosome component 3
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC002437
• Gene symbol: EXOSC3
• Gene ID (NCBI): 51010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA exosomes are multi-subunit complexes conserved throughout evolution, and they are emerging as the major cellular machinery for processing, surveillance and turnover of a diverse spectrum of coding and noncoding RNA substrates essential for viability[PMID:22544365]. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts [PMID:11782436]. EXOSC3 is a non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of Rnase PH-domain subunits through contacts with EXOSC9 and EXOSC5 [PMID:21255825].