EXOSC7 Polyklonaler Antikörper

EXOSC7 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 25292-1-AP

Synonyme

EAP1, EXOSC7, exosome component 7, hRrp42p, KIAA0116, p8, RRP42, Rrp42p

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 25292-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25292-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25292-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25292-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 25292-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25292-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25292-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25292-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25292-1-AP (EXOSC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 25292-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25292-1-AP (EXOSC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 25292-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 25292-1-AP (EXOSC7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A431-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

25292-1-AP bindet in WB, IP, IHC, IF, ELISA EXOSC7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXOSC7 fusion protein Ag18212
• Vollständiger Name: exosome component 7
• Berechnetes Molekulargewicht: 291 aa, 32 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC012831
• Gene symbol: EXOSC7
• Gene ID (NCBI): 23016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA

Publikationen

Species	Application	Title
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
human	WB,IF,IP	Cell Rep; ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells; Authors - M Carmen Lafita-Navarro

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (12-04-2022)

40µg of total protein were resolved on the gel. Incubation was done ON at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

EXOSC7 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:25292-1-AP)