EXOSC9 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

EXOSC9 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F4G1

Kat-Nr. : 67636-1-PBS

Synonyme

3F4G1, Exosome complex component RRP45, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, PM/Scl-75, PMSCL1

---

• 

  WB analysis using 67636-1-Ig (same clone as 67636-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67636-1-Ig (EXOSC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

  WB result of EXOSC9 antibody (67636-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC9 transfected HeLa cells. This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67636-1-Ig (EXOSC9 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67636-1-Ig (same clone as 67636-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC9 antibody (67636-1-Ig, Clone: 3F4G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67636-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP51449-1

  Cytometric bead array sample test of MP51449-1, EXOSC9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67636-1-PBS. Detection antibody: 67636-2-PBS.

• 

  Cytometric bead array standard curve of MP51449-1

  Cytometric bead array standard curve of MP51449-1, EXOSC9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67636-1-PBS. Detection antibody: 67636-2-PBS. Standard:Ag19783. Range: 3.125-400 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

67636-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA EXOSC9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EXOSC9 fusion protein Ag19783
• Vollständiger Name: exosome component 9
• Berechnetes Molekulargewicht: 456 aa, 51 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC142978
• Gene symbol: EXOSC9
• Gene ID (NCBI): 5393
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EXOSC9, also named as PMSCL1 and PM/Scl-75, is a key subunit of the exosome complex. EXOSC9 maintains self-renewal in a cell-autonomous manner. EXOSC9 has 4 isoforms with MW 49 kDa, 51 kDa, 39 kDa and 41 kDa. It's polymyositis/scleroderma auto-antigen 75 kDa. The MW of EXOSC9 is migrated to 60-75 kDa for modification in WB detection.