EXTL2 Polyklonaler Antikörper

EXTL2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12828-1-PBS

Synonyme

Alpha GalNAcT EXTL2, Alpha-1,4-N-acetylhexosaminyltransferase EXTL2, Alpha-GalNAcT EXTL2, EC:2.4.1.223, exostoses (multiple) like 2

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  WB analysis using 12828-1-AP (same clone as 12828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12828-1-AP (EXTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12828-1-AP (same clone as 12828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12828-1-AP (same clone as 12828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 12828-1-AP (same clone as 12828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12828-1-AP (same clone as 12828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12828-1-AP (EXTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 12828-1-AP (same clone as 12828-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using EXTL2 antibody (12828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12828-1-AP (same clone as 12828-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EXTL2 antibody (12828-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12828-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12828-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EXTL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXTL2 fusion protein Ag3548
• Vollständiger Name: exostoses (multiple)-like 2
• Berechnetes Molekulargewicht: 330 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37-43 kDa
• GenBank-Zugangsnummer: BC036015
• Gene symbol: EXTL2
• Gene ID (NCBI): 2135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Exostosin-like 2 (EXTL2) is one of three EXT-like genes in the human genome that are homologous to EXT1 and EXT2, encodes a transferase that adds not only GlcNAc but also N-acetylgalactosamine to the glycosaminoglycan (GAG)-protein linkage region via an α1,4-linkage. Loss of the enzyme EXTL2, a regulatory mechanism serving to limit CSPG synthesis, resulted in more extensive versican deposition into a lysolecithin-induced demyelinated injury, a larger area of myelin disruption with an associated decrease in axonal density within the lesion, and greater accumulation of macrophages and microglia (PMID: 32703234, 23395820)