EXTL3 Polyklonaler Antikörper

EXTL3 Polyklonal Antikörper für IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 10588-1-AP

Synonyme

EC:2.4.1.223, Exostosin-like 3, EXTL1L, EXTR1, EXT-related protein 1

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  IP experiment of mouse pancreas using 10588-1-AP

  IP result of anti-EXTL3 (IP:10588-1-AP, 4ug; Detection:10588-1-AP 1:1000) with mouse pancreas tissue lysate 3200 ug.

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  IP experiment of HEK-293T using 10588-1-AP

  IP result of anti-EXTL3 (IP:10588-1-AP, 4ug; Detection:10588-1-AP 1:1000) with HEK-293T cells lysate 1840 ug.

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Geprüfte Anwendungen

• Erfolgreiche IP: HEK-293T-Zellen, Maus-Pankreasgewebe

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

10588-1-AP bindet in WB, IF, IP, ELISA EXTL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EXTL3 fusion protein Ag0936
• Vollständiger Name: exostoses (multiple)-like 3
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC006363
• Gene symbol: EXTL3
• Gene ID (NCBI): 2137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Exostosin like glycosyltransferase 3 (EXTL3), also known as RPR, is located at chromosome 8p21.1 and composed of 919 amino acids, making it the longest member of the EXT family (PMID: 35676258). EXTL3 encodes the glycosyltransferases responsible for the biosynthesis of the backbone structure of heparan sulfate (HS) (PMID: 24128412). EXTL3 has been identified as a receptor molecule for regenerating islet-derived (REG) protein ligands, which have been shown to stimulate islet β-cell growth. Mutations in EXTL3 could lead to skeletal dysplasia, developmental delay, and severe immune deficiency (PMID: 28148688). The predicted molecular weight is 105 kDa, but we detected the band at 65 kDa perhaps due to alternative splicing (PMID: 14618397).

Publikationen

Species	Application	Title
human	WB,IF	Endocr Pract; Discovery of a human peptide sequence signaling islet neogenesis.; Authors - Levetan Claresa S CS
mouse,human	WB,IF	Ann N Y Acad Sci; REG1A protects retinal photoreceptors from blue light damage; Authors - Ze-Hua Xu