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EYA2 Polyklonaler Antikörper

EYA2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11314-1-PBS

Synonyme

EC:3.1.3.48, EAB1

Filter:
  • Western Blot (WB) analysis of various lysates using EYA2 Polyclonal antibody (11314-1-AP)

    WB analysis using 11314-1-AP (same clone as 11314-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11314-1-AP (EYA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11314-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EYA2 Polyclonal antibody (11314-1-AP)

    IHC staining of human prostate cancer using 11314-1-AP (same clone as 11314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11314-1-AP (EYA2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11314-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EYA2 Polyclonal antibody (11314-1-AP)

    IF Staining of HeLa using 11314-1-AP (same clone as 11314-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA2 antibody (11314-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11314-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11314-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EYA2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EYA2 fusion protein Ag1860
Vollständiger Name eyes absent homolog 2 (Drosophila)
Berechnetes Molekulargewicht 64 kDa
Beobachtetes Molekulargewicht56 kDa and 70 kDa
GenBank-ZugangsnummerBC013882
Gene symbol EYA2
Gene ID (NCBI) 2139
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EYA2, also named EAB1, belongs to the HAD-like hydrolase superfamily and EYA family. It coactivates SIX1. The Six1-EYA2 interaction may inhibit breast cancer progression. EYA2 is required for Six1-activated TGF-beta signaling.(PMID:21706047) It may be involved in the development of the eye. This protein has 3 isoforms produced by alternative splicing with the molecular weight of 59 kDa, 57 kDa, and 50 kDa. It can be detected two bands of 70 kDa and 56 kDa in the neuroblastoma cells by western blot(PMID:12039049).