EZH2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

EZH2 Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1F10A12

Kat-Nr. : 66476-1-Ig

Synonyme

Enhancer of zeste homolog 2, ENX 1, ENX-1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6

---

Galerie der Validierungsdaten

• 

  WB analysis using 66476-1-Ig

  Various cell lines were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66476-1-Ig

  WB result of EZH2 antibody (66476-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EZH2 transfected HEK-293 cells.

• 

  WB analysis using 66476-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66476-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66476-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66476-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 66476-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66476-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 66476-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EZH2 (66476-1-Ig, Clone:1F10A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, 4T1-Zellen, A431-Zellen, A549-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-3-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

66476-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA EZH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EZH2 fusion protein Ag16789
• Vollständiger Name: enhancer of zeste homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 751 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-102 kDa
• GenBank-Zugangsnummer: BC010858
• Gene symbol: EZH2
• Gene ID (NCBI): 2146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; Long noncoding RNA MEG3 regulates LATS2 by promoting the ubiquitination of EZH2 and inhibits proliferation and invasion in gallbladder cancer.; Authors - Longyang Jin
human	WB,IHC	Front Cell Dev Biol; Exosome-Delivered LncHEIH Promotes Gastric Cancer Progression by Upregulating EZH2 and Stimulating Methylation of the GSDME Promoter.; Authors - Yan Lu ; KD Validated
mouse	WB	Front Cell Dev Biol; Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery; Authors - Nicholas Marano
human	WB	Cancer Med; LncRNA SPRY4-IT1 facilitates cell proliferation and angiogenesis of glioma via the miR-101-3p/EZH2/VEGFA signaling axis; Authors - Ji Wang
mouse	WB,IP	Int Immunopharmacol; Celastrol attenuates diabetic nephropathy by upregulating SIRT1-mediated inhibition of EZH2related wnt/β-catenin signaling; Authors - Yuewen Tang
	IF	Bioact Mater; Engineered mesenchymal stem cell-derived small extracellular vesicles for diabetic retinopathy therapy through HIF-1α/EZH2/PGC-1α pathway; Authors - Fengtian Sun